
Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568
Rat IgG 검출용 고순도 Donkey 유래 2차 항체로 Alexa Fluor 568 형광 표지. 다양한 면역실험(WB, ICC/IF, Flow)에 사용 가능. 광범위한 종 교차흡착으로 높은 특이성과 낮은 배경 신호 제공. 안정적 형광 신호와 높은 감도 유지.
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Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 568 |
| Excitation/Emission Max | 579/603 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient |
| RRID | AB_2910653 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep serum |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- HRP
- TRITC
- Request custom conjugation available
Product Specific Information
This donkey anti-rat IgG (H+L) whole secondary antibody is affinity-purified and exhibits minimal cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep serum proteins.
Cross-adsorption enhances antibody specificity, improving sensitivity and reducing background staining. This step is particularly beneficial in multiplex or multicolor staining experiments such as flow cytometry or fluorescence imaging.
Alexa Fluor 568 dye is a bright orange/red fluorophore with excitation ideally suited to the 568 nm laser line. It provides stable, pH-insensitive fluorescence with high quantum yield and photostability. The typical labeling ratio is 2–8 fluorophore molecules per IgG molecule.
Before use, briefly centrifuge the conjugate solution and use only the supernatant to avoid aggregates. Recommended working concentration for most applications is 1–10 µg/mL.
Product is shipped at room temperature.
Target Information
Anti-Rat secondary antibodies are affinity-purified reagents specific for rat immunoglobulins. They are useful for detection, sorting, and purification of rat antibodies. Secondary antibodies enhance assay sensitivity via signal amplification and are compatible with multiple detection systems (HRP, AP, fluorescence).
Produced by immunizing host animals with pooled rat immunoglobulins and purified via immunoaffinity chromatography.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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