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Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568
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Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

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Rat IgG 검출용 고순도 Donkey 유래 2차 항체로 Alexa Fluor 568 형광 표지. 다양한 면역실험(WB, ICC/IF, Flow)에 사용 가능. 광범위한 종 교차흡착으로 높은 특이성과 낮은 배경 신호 제공. 안정적 형광 신호와 높은 감도 유지.

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A78946
판매단위
pk
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마지막 업데이트 2025. 08. 03. 오전 09:25
Thermo Fisher Scientific A78946 Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 1 mg pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Applications and Tested Dilution

Application Tested Dilution
Western Blot (WB) 0.5–2 µg/mL
Immunocytochemistry (ICC/IF) 1–2 µg/mL
Flow Cytometry (Flow) 1–4 µg/mL

Product Specifications

Specification Description
Species Reactivity Rat
Host/Isotype Donkey / IgG
Class Polyclonal
Type Secondary Antibody
Immunogen Gamma Immunoglobulins Heavy and Light chains
Conjugate Alexa Fluor™ 568
Excitation/Emission Max 579/603 nm
Form Liquid
Concentration 2 mg/mL
Purification Purified
Storage Buffer PBS, pH 7.5
Contains 5 mM sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient
RRID AB_2910653
Target IgG
Cross Adsorption Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep serum
Antibody Form Whole Antibody

Additional Formats Available

  • Alexa Fluor 488
  • Alexa Fluor 555
  • Alexa Fluor 594
  • Alexa Fluor 647
  • Alexa Fluor Plus 405
  • Alexa Fluor Plus 488
  • Alexa Fluor Plus 555
  • Alexa Fluor Plus 594
  • Alexa Fluor Plus 647
  • AP
  • Biotin
  • FITC
  • HRP
  • TRITC
  • Request custom conjugation available

Product Specific Information

This donkey anti-rat IgG (H+L) whole secondary antibody is affinity-purified and exhibits minimal cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep serum proteins.
Cross-adsorption enhances antibody specificity, improving sensitivity and reducing background staining. This step is particularly beneficial in multiplex or multicolor staining experiments such as flow cytometry or fluorescence imaging.

Alexa Fluor 568 dye is a bright orange/red fluorophore with excitation ideally suited to the 568 nm laser line. It provides stable, pH-insensitive fluorescence with high quantum yield and photostability. The typical labeling ratio is 2–8 fluorophore molecules per IgG molecule.

Before use, briefly centrifuge the conjugate solution and use only the supernatant to avoid aggregates. Recommended working concentration for most applications is 1–10 µg/mL.

Product is shipped at room temperature.

Target Information

Anti-Rat secondary antibodies are affinity-purified reagents specific for rat immunoglobulins. They are useful for detection, sorting, and purification of rat antibodies. Secondary antibodies enhance assay sensitivity via signal amplification and are compatible with multiple detection systems (HRP, AP, fluorescence).
Produced by immunizing host animals with pooled rat immunoglobulins and purified via immunoaffinity chromatography.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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