Thermo Fisher Scientific KT3 tag Polyclonal Antibody
KT3 태그 단백질 검출용 토끼 폴리클로날 항체. WB 및 ELISA에 최적화된 고감도 항체로, 항원 친화 크로마토그래피로 정제됨. 세포 성장 및 생존 관련 연구에 활용 가능. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 0.2% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2902976 |
Product Specific Information
This product is predicted to work in ELISA at a dilution of 1:10,000.
Target Information
The KT3 tag does not correspond to a specific gene but is often used in biochemical and molecular biology research as a peptide tag for protein detection and purification. It is related to the PI3K/AKT/mTOR signaling pathway, which is a critical regulatory network in eukaryotic cells involved in cell survival, growth, and metabolism. The PI3K (phosphatidylinositol 3-kinase) enzyme generates phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3), acting as a second messenger that facilitates the translocation and activation of Akt at the plasma membrane by phosphoinositide-dependent kinases. This pathway is frequently activated in various cancers, contributing to tumorigenesis and cancer progression by promoting cell growth and inhibiting apoptosis. Targeted therapies inhibiting this pathway are being studied for their potential in cancer treatment.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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