Thermo Scientific™

EGS (ethylene glycol bis(succinimidyl succinate))

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be자세히 알아보기

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Features of the EGS crosslinker:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
• Membrane-permeable, allowing for intracellular crosslinking
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EGS (ethylene glycol bis(succinimidyl succinate))

사양

화학물질 반응성Amine-Amine

CleavableBy Hydroxylamine

설명EGS

분자량456.36

페길레이티드No

스페이서 암 길이16.1 Å

세포 투과성Yes

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Homobifunctional

반응성 부분NHS Ester

스페이서Medium (10 to 30 Å)

형태Powder

수량1 g

용해도DMF, DMSO

형식Standard

수용성No

제품 유형Crosslinker

Unit SizeEach