Thermo Fisher Scientific DTSSP (3,3`-dithiobis(sulfosuccinimidyl propionate))
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
21578 | - | Thermo Fisher Scientific 21578 DTSSP (3,3`-dithiobis(sulfosuccinimidyl propionate)) Each pk | 재고문의 | pk | 224,000원 | - | 246,400원 |
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Thermo Scientific™
DTSSP (3,3`-dithiobis(sulfosuccinimidyl propionate))
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose자세히 알아보기
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
DTSSP is 3,3`-Dithiobis(sulfosuccinimidylpropionate), which contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker.
Features of DTSSP:
• Reactive groups: sulfo-NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm also easily cleaved with reducing SDS-PAGE sample loading
• Cleavable crosslinker allows separation of crosslinked products
• Water-soluble; compare with DSP
• Membrane-impermeable, allowing for cell surface labeling
Applications:
• Chemical crosslinking of cell surface proteins prior to cell lysis and immunoprecipitation
• Fix
protein interactions to allow identification of weak or transient protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces
Properties of DTSSP
• Alternative names: Sulfo-DSP
• Molecular weight: 608.51
• Spacer arm length: 12.0 Å (8 atoms)
• Reactive groups: Sulfo-NHS esters, react with primary amines at pH 7.0–9.0
DTSSP Specifications
We manufacture DTSSP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of DTSSP is tested to meet the following minimum specifications:
• Solubility: > 6 mg/mL in DI water
• Identity: IR scan shows only peaks characteristic of the structure and functional groups of DTSSP
• Purity: > 80% by quantitative NMR (the highest standard for crosslinker purity)
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
DTSSP (3,3-dithiobis(sulfosuccinimidyl propionate))](https://www.thermofisher.com/order/catalog/product/21578)
[DSP (dithiobis(succinimidyl propionate)), Lomant
s Reagent
DSS (disuccinimidyl suberate)
사양
화학물질 반응성Amine-Amine
CleavableBy Thiols
설명DTSSP
분자량608.51
페길레이티드No
스페이서 암 길이12.0 Å
세포 투과성No
배송 조건Ambient
제품라인Pierce™
라벨링 방법Chemical Labeling
교차결합제 유형Homobifunctional
반응성 부분Sulfo-NHS Ester
스페이서Medium (10 to 30 Å)
형태Powder
수량50 mg
용해도Water
형식Standard
수용성Yes
제품 유형Crosslinker
Unit SizeEach
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