Thermo Fisher Scientific LEF1 Monoclonal Antibody (1C3.1D10)
LEF1 단백질을 인식하는 Mouse monoclonal 항체로, WB, ICC/IF, ChIP assay에 사용 가능. TCF-1 및 TCF-4와 교차 반응 없음. Wnt 신호전달 관련 연구 및 암 발현 분석에 적합. 단백질 A/G로 정제된 액상 형태.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ChIP assay (ChIP) | 2.5 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C3.1D10 |
| Immunogen | Recombinant full length LEF-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS |
| Contains | 0.08% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1076477 |
Product Specific Information
This antibody does not cross-react with TCF-1 or TCF-4.
Target Information
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity.
This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis.
Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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