Thermo Fisher Scientific Ki-67 Recombinant Rabbit Monoclonal Antibody (MKI67, 4947R)
인간 Ki-67 단백질을 인식하는 재조합 토끼 단클론 항체로, 세포 증식 연구에 사용됩니다. IHC(P) 검증 완료, 고순도 Protein A/G 정제, 4°C 보관, 형광 또는 효소 비접합(Unconjugated) 형태로 제공됩니다.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MKI67, 4947R |
| Immunogen | Recombinant fragment (around aa 2293–2478) of human Ki67 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, then cool at room temperature for 20 min.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Expression patterns vary by cell cycle stage — perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
Ki-67 serves as a proliferation marker, absent in G0 cells but increasing from G1 through mitosis. It is widely used to assess the growth fraction in neoplasms, correlating with tumor grade and prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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