Thermo Fisher Scientific API5 Polyclonal Antibody
Human, Mouse, Rat 반응성의 Rabbit Polyclonal Antibody로, Western Blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관됩니다. API5 단백질을 표적으로 하며 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human API5. Recombinant protein control fragment (Product #RP-92069). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.08 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638070 |
Product Specific Information
Immunogen sequence:
TVEELYRNYG ILADATEQVG QHKDAYQVIL DGVKGGTKEK RLAAQFIPKF FKHFPELADS AINAQLDLCE DEDVSIRRQA IKELPQFATG ENLPRVADIL TQLLQTDDSA EFNLVN
Highest antigen sequence identity to the following orthologs:
Mouse – 100%, Rat – 100%.
Target Information
This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. It suppresses E2F1-induced apoptosis and interacts with Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Depletion enhances the cytotoxic action of chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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