Thermo Fisher Scientific alpha Actinin 2/3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized peptide derived from N-terminal of human Actin alpha-2/3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Actinin 2/3,uniProtId:P35609-1,ncbiNodeId:9606,antigenRange:1,antigenLength:894,antigenImageFileName:PA5-49802_alpha_Actinin_23_P35609-1_Rabbit.svg,antigenImageFileNamePDP:PA5-49802_alpha_Actinin_23_P35609-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:alpha Actinin 2/3,uniProtId:Q08043-1,ncbiNodeId:9606,antigenRange:1,antigenLength:901,antigenImageFileName:PA5-49802_alpha_Actinin_23_Q08043-1_Rabbit.svg,antigenImageFileNamePDP:PA5-49802_alpha_Actinin_23_Q08043-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2635255

Product Specific Information

The antibody detects endogenous levels of total alpha Actinin 2 + alpha Actinin 3 protein.

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.