Gibco™

DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

DMEM (Dulbecco`s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기

DMEM (Dulbecco`s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• HEPES
• Sodium Pyruvate
• GlutaMAX™
• Phenol Red

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle`s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco™ DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical DMEM product made in our Scotland facility (31966-021). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

사양

세포주HeLa, 293, Cos-7, and PC-12

세포 유형Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

분류Animal Origin-free

농도1 X

형태Liquid

제품 유형DMEM (Dulbecco`s Modified Eagle Medium)

Serum LevelStandard Serum Supplementation

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate

첨가제 없음No HEPES

용도(애플리케이션)Mammalian cell culture

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™, GlutaMAX™

수량500 mL

배송 조건Room Temperature

Unit SizeEach