Thermo Fisher Scientific TPL2 Polyclonal Antibody
Thermo Fisher Scientific의 TPL2 Polyclonal Antibody는 인간, 마우스, 랫트에서 반응하며 Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체로, MAP3K8 신호 전달 연구에 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 250–300 of Human Cot |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2802924 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
MAP3K8 (COT) is a serine/threonine kinase that activates both MAP and JNK kinase pathways and plays a role in the cell cycle.
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase (MAPKKK or MEKK).
MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK.
These kinases are highly conserved across species, including yeast, Drosophila, and mammals.
MEKK8 activates NF-kappa-B 1 via proteasome-mediated proteolysis of NF-kappa-B 1/p105.
This cytoplasmic protein is expressed in various normal tissues and tumor-derived cell lines.
The 58 kDa form is activated during the S and G2/M phases of the cell cycle.
The longer form undergoes phosphorylation mainly on Ser residues, and the shorter form on both Ser and Thr residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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