Thermo Fisher Scientific NUMB Polyclonal Antibody
NUMB 단백질을 인식하는 염소 유래 폴리클로날 항체로, 인간 및 생쥐에 반응합니다. Western blot, IHC, ICC, Flow Cytometry에 사용 가능합니다. 합성 펩타이드 면역원 기반으로 제작되었으며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.01–0.03 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 6–8 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (PFSSDLQKTFEIEL) corresponding to the C-terminus amino acids of NUMB (aa 638–651) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10982036 |
Product Specific Information
- Predicted to react with bovine and canine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that regulates the differentiation of neuronal progenitor cells. It functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain show extensive neurite outgrowth, which can be inhibited by voltage-gated Ca²⁺ channel inhibitors or Ca²⁺ chelators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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