Thermo Fisher Scientific BCKDE1A Polyclonal Antibody
Thermo Fisher Scientific의 BCKDE1A 폴리클로날 항체는 인간 BCKDHA 단백질(75–125 aa)을 면역원으로 제작된 Rabbit IgG 항체입니다. Western blot 및 Immunoprecipitation에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 1 mg/mL 농도, 액상 형태, 4°C 보관.
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Applications
Western Blot (WB)
- Assay-dependent
Immunoprecipitation (IP)
- 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 75 and 125 of human Branched chain keto acid dehydrogenase E1, alpha polypeptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4).
This inner-mitochondrial enzyme complex catalyzes the oxidative decarboxylation of branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine — the second major step in BCAA catabolism (Wynn et al., 1998).
The BCKD complex consists of:
- A heterotetrameric (α2-β2) branched-chain α-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase (E2)
- A homodimeric dihydrolipoamide dehydrogenase (E3)
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme, and the reaction is irreversible — constituting the first committed step in BCAA oxidation.
The BCKDHB gene encodes the beta subunit of E1.
The complex also contains two regulatory enzymes: a kinase and a phosphorylase.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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