Thermo Fisher Scientific Human CXCL12 (SDF-1g) Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKGR REEKVGKKEK IGKKKRQKKR KAAQKRKN

Molecular weight

11.6 kDa

Class

Recombinant

Type

Protein

Purity

≥98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human T cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human CXCL12 (SDF-1g) is a 11.6 kDa protein containing 98 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The SDF-1 proteins are stromal-derived CXC chemokines that signal primarily through the CXCR4 receptor. SDF-1 proteins exert chemotactic activity over a broad range of immune system cell types and may also be involved in other processes including metastasis of certain cancers, inflammation, and angiogenesis. Additionally, SDF-1 can suppress HIV entry into cells expressing the CXCR4 receptor. Five isomers of SDF-1 have been found with SDF-1alpha being the predominant form, expressing in a wide range of cell types. The SDF-1gamma isoform is distinctive in that it contains a highly basic C-terminal region, which presumably is the reason for its high binding affinity to gylcosaminoglycans (GAG) and heparin sulfate (HS). Consequently, most of SDF-1gamma exists in a cell- and membrane-bound form, which confers greater stability compared to other isoforms via increased resistance to proteolytic degradation. SDF-1gamma binds with reduced affinity to the CXCR4 receptor when compared to the alpha and beta isoforms, but because of its increased stability can, over time, exert equal or greater activity when compared to other SDF-1 isoforms. SDF-1gamma expression has been found primarily in the heart (human and mouse) and in the brain and central nervous system (rat).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.