Thermo Fisher Scientific ACBD3 Monoclonal Antibody (OTI3A1), TrueMAB
ACBD3 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, IHC, Flow Cytometry에 적합합니다. 인간, 개, 랫드에서 반응하며, 동결건조 형태로 제공됩니다. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 보장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A1 |
| Immunogen | Full length human recombinant protein of human ACBD3 produced in HKE293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
GOLPH1 (also known as GCP60) is a Golgi-associated protein that interacts with the integral membrane protein giantin and contributes to Golgi structure maintenance. It also interacts with Golgi proteins such as Golgin-160, which can be cleaved by caspases, leading to nuclear localization of its fragments.
The interaction strength between GOLPH1 and Golgin-160 fragments is regulated by the oxidation state of Cys-463, indicating a coordinated process in nuclear localization.
Additionally, GOLPH1 interacts with Numb, a signaling protein involved in asymmetric cell division, suggesting that Golgi dynamics may influence cell fate and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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