Thermo Fisher Scientific RACGAP1 Monoclonal Antibody (OTI3D8), TrueMAB
RACGAP1 단백질을 인식하는 Mouse monoclonal 항체로, Western blot과 IHC(Paraffin)에 최적화됨. Human, Mouse, Rat 시료에 반응하며, 동결건조 형태로 제공. PBS buffer에 trehalose 포함, -20°C 보관 권장.
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- CF811651
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- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 123–238 of human RACGAP1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia, and filopodia.
It is involved in cytoskeletal actin organization and cellular transformation.
The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42, inducing assembly of polarized cytoskeletal structures in fibroblasts.
RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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