Thermo Fisher Scientific RACGAP1 Monoclonal Antibody (OTI1C10), TrueMAB
RACGAP1 단백질(아미노산 123–238)을 항원으로 한 Mouse IgG1 단클론 항체. Western blot에 적합하며 Human, Mouse, Rat 등 다양한 종에 반응. 동결건조 형태로 제공되며, PBS와 trehalose buffer 포함. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 123–238 of human RACGAP1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve ~1 mg/mL final concentration.
- For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia, and filopodia. It is involved in cytoskeletal actin organization and transformation.
The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42, which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts.
RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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