Thermo Fisher Scientific CBX5 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Chromobox Homolog 5 (HP1 alpha). Validated for WB, IHC, IHC-P, and IP applications. Unconjugated liquid form, concentration 1 mg/mL. Recommended storage at 4°C, shelf life 1 year.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 (HP1 alpha, UniProt ID: P45973-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, mediating gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules involved in gene silencing and chromatin structure. High-affinity recognition of methylated histone H3 by HP1 requires a functional chromodomain, which interacts specifically with the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-knockout mouse fibroblasts but restored after reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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