Thermo Fisher Scientific CBX5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (IHC)

1:2,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,000-1:10,000

-

Immunoprecipitation (IP)

2-5 µg/mg lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HP1 alpha,uniProtId:P45973-1,ncbiNodeId:9606,antigenRange:150-191,antigenLength:191,antigenImageFileName:A300-877A_HP1_alpha_P45973-1_Rabbit.svg,antigenImageFileNamePDP:A300-877A_HP1_alpha_P45973-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double- primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.