Thermo Fisher Scientific Phosphotyrosine (P-Tyr) Monoclonal Antibody (PY265)
Tyrosine 인산화 단백질 검출용 단클론 항체. Western blot, ICC/IF, Flow cytometry에 적합. Mouse IgG1, kappa 형식. 4°C 보관, 동결 금지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PY265 |
| Immunogen | Phosphotyrosine conjugated to BSA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Tyrosine phosphorylation plays a key role in transducing mitogenic signals from transmembrane receptors and in oncogene tyrosine kinase–mediated transformation. It is a primary mechanism in signal transduction during normal mitogenesis, cell cycle progression, and oncogenic transformation. Its involvement in differentiation and gap junction communication remains under investigation.
Antibodies that specifically recognize phosphorylated tyrosine residues are invaluable for studying tyrosine-phosphorylated proteins and related biochemical pathways. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is particularly useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical protocols, especially when the use of a secondary antibody would complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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