Thermo Fisher Scientific Click-iT EdU Imaging Kit with Alexa Fluor 488, 594, and 647 Azides

Invitrogen™ Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488, 594, and 647 Azides

The Click-iT™ EdU Imaging Kit is a trial version of our superior alternative to traditional proliferation assays optimized for fluorescence microscopy applications. In this assay, the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.

This trial-size kit provides sufficient EdU to label up to 6–30 coverslips and enough Alexa Fluor™ 488, 594, and 647 azide to detect new DNA synthesis on 2–10 coverslips with each fluorophore.

주요 특징

• Simple — Works the first time, every time, in less time
• Efficient — No denaturation steps or harsh treatment required
• Content-rich results — Better preservation of cell morphology, antigen structure, and DNA integrity
• Consistent — Not dependent on variable antibody lots for detection

Superior Proliferation Methodology

The Click-iT™ EdU Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT™ EdU, mild fixation and detergent permeabilization are sufficient for the small molecule-based Click-iT™ EdU detection reagent to access DNA. As a result, the kit is easy to use, accurate, and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.

This kit is optimized for fluorescence microscopy applications. Visit the Click-iT™ technology area of our website for kits designed for high-content imaging, fluorescence microplate, or flow cytometry platforms.

Notes:

• The Click-iT™ assay can be used on cells in culture or in vivo following EdU administration by feeding or injection methods.
• The Click-iT™ assay can be used with BrdU in dual pulse experiments using the anti-BrdU (clone MoBu-1) antibody, which does not cross-react with EdU.
• Compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed cell labeling.

사양

제품 이미지