Thermo Fisher Scientific Human TEK (TIE2), GST Tag Recombinant Protein

Thermo Fisher Scientific Human TEK (TIE2), GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Spin prior to use for maximum recovery.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use original packaging until exhausted.
• For larger sizes, prepare aliquots to reduce freeze/thaw cycles (not below 20 µL).
• Do not store kinase diluted.
• Guaranteed for 6 months from date of purchase if properly stored at -80°C.
• Protein Form: Recombinant, Cytoplasmic
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Z'-LYTE™, Omnia™ Bulk Substrate

Target Information

The TEK receptor tyrosine kinase (also known as TIE2) is expressed predominantly in endothelial cells. TEK signaling is essential for endothelial cell–smooth muscle cell communication during venous morphogenesis, and mutations in TEK are linked to inherited venous malformations.
Angiopoietin-1 binds to TEK and activates a pathway crucial for embryonic vascular development. TIE2 expression increases with thyroid-stimulating hormone and agents that elevate intracellular cAMP.
Hematopoietic stem cells (HSCs) expressing TIE2 are quiescent, antiapoptotic, and form a side population adhering to osteoblasts in the bone marrow niche.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.