Thermo Fisher Scientific POLI Polyclonal Antibody
DNA 손상 복구 관련 POLI 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot 및 ICC/IF에 사용 가능. Human에 반응하며 Mouse, Rat 등에서도 예측 반응성 있음. 항원 친화 크로마토그래피로 정제된 액상 형태.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
- View 1 publication
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 122–396 of Human DNA polymerase iota (POLI, UniProt ID: Q9UNA4-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris Glycine (pH 7), with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546918 |
Product Specific Information
- Recommended positive controls: PC-3, HEK293
- Predicted reactivity: Mouse (88%), Rat (85%), Chimpanzee (98%), Bovine (89%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5’-deoxyribose phosphate at abasic sites, but may not have lyase activity.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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