Thermo Fisher Scientific Histone H3.3 Recombinant Rabbit Monoclonal Antibody (ARC0255)
Histone H3.3 단백질을 인식하는 재조합 토끼 단일클론 항체로, WB, IHC, ELISA, ChIP 및 ChIP-Seq에 최적화됨. HEK293 세포에서 발현되며 높은 순도와 안정성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 2 µg antibody for 5–10 µg of Chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:50–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0255 |
| Immunogen | Synthetic peptide corresponding to amino acids 57–136 of human Histone H3.3 (UniProt ID: P84243) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849487 |
Product Specific Information
Immunogen sequence:
KSTELLIRKL PFQRLVREIA QDFKTDLRFQ SAAIGALQEA SEAYLVGLFE DTNLCAIHAK RVTIMPKDIQ LARRIRGERA
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of eukaryotic chromosomal fibers. Nucleosomes consist of ~146 bp of DNA wrapped around a histone octamer composed of pairs of H2A, H2B, H3, and H4. The chromatin fiber is compacted through interaction with linker histone H1.
The gene encoding histone H3.3 is intronless and produces transcripts lacking polyA tails, containing instead a palindromic termination element. It is located separately from other H3 genes on chromosome 6p22–p21.3.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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