Thermo Scientific™

SYBR™ Green qPCR Master Mix

Thermo Scientific SYBR Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA자세히 알아보기

Thermo Scientific SYBR Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Features of Thermo Scientific SYBR Green qPCR Master Mix include:

• Broad instrument and workflow compatibility enabling SYBR Green qPCR Master Mix to be used with a wide variety of real-time PCR instruments and reverse transcriptase (RT) enzymes
• Highly sensitive and specific low-level detection of your target of interest
• Wide linear dynamic range—precise measurement across six logs of dynamic range, enabling accurate detection with both low- and high -concentration samples1

Broad instrument compatibility

Thermo Scientific SYBR Green qPCR Master Mix can be used in either standard or fast cycling mode and is compatible with a wide variety of real-time PCR instruments.

Broad workflow compatibility

Thermo Scientific SYBR Green qPCR Master Mix is compatible with a variety of RT enzymes and provides flexibility for varying primer melting temperatures (Tm), between 55°C and 65°C.

Highly sensitive and specific

Thermo Scientific SYBR Green qPCR Master Mix enables low-level detection and contains an antibody-mediated hot-start mechanism to mitigate non-specific early polymerase activity. See figure for a performance comparison vs. other leading master mixes.

Wide dynamic range

Thermo Scientific SYBR Green qPCR Master Mix can yield up to 6-log dynamic range for cDNA (see figure) and shows equivalent or better dynamic range in the detection of various targets compared to other master mixes.

Additional features

• Stability of pre-assembled reactions for up to 72 hours in dark room at room temperature2
• Reduce carryover contamination—uracil-DNA glycosylase (UDG) and dUTP included in mix to help ensure leftover DNA residue is not re-amplified in subsequent experiments

Footnotes
1. See user guide for specific sample concentration recommendations.
2. Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.

사양

검출 방법SYBR

형태Frozen

GC-Rich PCR PerformanceHigh

PCR 방법qPCR, 2-step RT-qPCR

반응 속도Fast or Standard

라벨 또는 염료SYBR Green

수량5 mL

용도(애플리케이션)Gene Expression, DNA Quantitation, Pathogen Detection, Microbial Detection

반응 수500 x 20 μL Reactions

Unit SizeEach