Thermo Fisher Scientific AgriSeq Genomic DNA Extraction Kit

Applied Biosystems™ AgriSeq™ Genomic DNA Extraction Kit

The AgriSeq Genomic DNA Extraction Kit enables rapid generation of PCR-ready genomic DNA for next-generation sequencing (NGS) and other applications from a variety of aquaculture and plant tissues in less than 10 minutes.
No bead beating or mechanical homogenization of tissues is required. After DNA extraction, NGS amplicon library preparation can be carried out using the AgriSeq HTS Library Kit workflow.

Features

• Simple and fast protocol — takes less than 10 minutes with few steps
• Universal — purifies genomic DNA from aquaculture and plant tissues
• No bead beating or mechanical homogenization required
• No phenol extraction
• Applications: NGS and qPCR

How to Use

1. Mix your tissue sample with the AgriSeq Genomic DNA Extraction lysis solution provided.
2. Perform direct lysis by incubating for 2–5 minutes on a thermal cycler at 95–97°C for cell lysis.
3. Lower the temperature to 25°C for 3 minutes and add the AgriSeq Genomic DNA stabilizing solution to complete the crude lysis isolation.
4. Spin the plate briefly in a centrifuge to separate tissue residue and lysate.
5. Store crude lysate at 4°C (short-term) or –20°C (long-term), or proceed directly to PCR amplification.

Note: For NGS applications, add 1 μL of Plant RNA Isolation Aid per 10 μL total reaction volume in place of nuclease-free water.
For example, in a 25 μL reaction volume, replace 2.5 μL of water with 2.5 μL RNA Isolation Aid.

Specifications

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