Applied Biosystems™

AgriSeq™ Genomic DNA Extraction Kit

The AgriSeq Genomic DNA Extraction Kit enables generation of PCR-ready genomic DNA for next-generation sequencing and other applications from a자세히 알아보기

The AgriSeq Genomic DNA Extraction Kit enables generation of PCR-ready genomic DNA for next-generation sequencing and other applications from a variety of aquaculture and plant tissues in less than 10 minutes. No bead beating or mechanical homogenization of tissues is required. After DNA extraction, NGS amplicon library prep can be carried out using the AgriSeq HTS Library kit workflow.

Features of the AgriSeq Genomic DNA Extraction Kit include:

• Simple and fast protocol—takes less than 10 mins with few steps
• Universal—purifies genomic DNA from aquaculture and plant tissues
• No bead beading or mechanical homogenization of tissues
• Does not involve phenol extraction
• Applications: NGS and qPCR

How to use

Simply mix your tissue sample with the AgriSeq Genomic DNA Extraction lysis solution provided. Perform direct lysis by incubating for 2–5 minutes on a thermal cycler at 95–97°C for cell lysis. After lysis lower the temperature to 25°C for 3 minutes and add the AgriSeq Genomic DNA stabilizing solution to complete the crude lysis isolation. Spin the plate down briefly in the centrifuge to separate the tissue residue and the lysate. Store crude lysate at 4°C short-term or –20°C long-term or proceed directly to PCR amplification.

Note: For NGS applications, add 1 μL of Plant RNA Isolation Aid per 10 μL total reaction volume in place of nuclease-free water. For example, in a 25 μL reaction volume, replace 2.5 μL of water with 2.5 μL RNA Isolation Aid.

사양

형식96-well Plate

샘플 종류Leaf Punches, Seed Chips, Tissue pieces, Fin Punches, Plieopods

용도(애플리케이션)Genomic DNA extraction using a simple direct cell lysis protocol for downstream NGS and qPCR applications

반응 수9600 Reactions

핵산 유형DNA

최적 반응 온도95°C to 97°C (Lysis initiation), 25°C (Lysis incubation)

PCR 방법Multiplex Amplicon PCR

플레이트 호환성MicroAmp™ EnduraPlate™ Optical 96-well Clear Reaction Plates

플레이트 유형Deep-well

제품라인AgriSeq™

제품 유형Genomic DNA Extraction Kit

수량9600 reactions

배송 조건Cool Gel Pack

유통 기한14 Months

종Plants, Aquaculture

워크플로우 단계DNA Extraction

샘플 소스Plants: leaf, seed, or tissue; Aquaculture: Fins, Plieopods

시작 물질 양2 x 3 mm (or 1 x 5 to 6 mm) leaf/fin punch

Unit SizeEach