Thermo Fisher Scientific LEF1 Polyclonal Antibody
LEF1 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체. Western blot, ICC/IF, Flow Cytometry에 적합. 인간 시료에 반응하며, Wnt 신호전달 연구에 유용. 액상 형태로 -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (QHEQRKEQEPKRPH) corresponding to the internal amino acids of LEF1 (aa 285–299). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10989233 |
Product Specific Information
- This antibody is predicted to react with canine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
The LEF1 gene encodes a transcription factor belonging to the high mobility group (HMG) protein family.
This protein binds to a functional site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity.
It participates in the Wnt signaling pathway and may play roles in hair cell differentiation and follicle morphogenesis.
Mutations in this gene have been identified in sebaceous tumors and are associated with various cancers, including androgen-independent prostate cancer.
Alternative splicing results in multiple transcript variants.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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