Thermo Fisher Scientific Septin-8 Polyclonal Antibody
Rabbit polyclonal antibody targeting Septin-8 protein for WB, IHC, ICC applications. Reacts with human, mouse, and rat. High specificity with antigen affinity purification. Recommended controls include HCT116 and brain tissues. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | – |
| Immunohistochemistry (IHC) | – | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 174–458 of Human SEPT8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.83 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2548830 |
Product Specific Information
- Recommended positive controls: HCT116, mouse brain, rat brain
- Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (87%), Rabbit (96%), Chicken (90%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SEPT8 is a member of the highly conserved septin family. Septins are 40–60 kDa GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, neuronal polarity, and vesicle trafficking (Blaser et al., 2003 [PubMed 12909369]).
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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