Thermo Fisher Scientific NSFL1C Polyclonal Antibody
NSFL1C 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ELISA, IP에 적합합니다. Human, Mouse, Rat 시료에 반응하며, Affinity Chromatography로 정제된 고순도 항체입니다. PBS/glycerol buffer에 보관되며 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–185 of human NSFL1C (NP_057227.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.49 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914596 |
Product Specific Information
Positive test controls include: 293T, U-251MG, Mouse kidney, Mouse brain, Rat brain, Rat kidney.
The target is usually found in the following locations: Chromosome, Golgi apparatus, Golgi stack, Nucleus.
Immunogen sequence:
MAAERQEALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEDEEEEE GQRFYAGGSE RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF AGGGYRLGAA PEEESAYVAG EKRQHSSQDV HVVLK
Target Information
p47 (also known as NSFL1C, UBX1, UBXD10, or UBXN2C) is a 370-amino acid protein localized to both the nucleus and the Golgi apparatus (specifically Golgi stacks).
It contains one SEP domain and one UBX domain and functions as part of a ternary complex with VCP and Syntaxin 5, regulating vesicle fusion and Golgi stack reassembly during mitosis.
Phosphorylation of p47 during mitosis inhibits Golgi interaction, essential for proper Golgi stack formation.
Human p47 shares 89% sequence identity with the mouse counterpart, indicating conserved function across species.
Multiple isoforms exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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