Thermo Fisher Scientific ATM Monoclonal Antibody (5C2)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: Raji, HeLa nuclear extract.
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family.
Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and increased risk of lymphoid cancer.

The ATM gene encodes a protein kinase that phosphorylates key regulators of the cell cycle including p53, Mdm2, BRCA1, and SMC1.
ATM plays a critical role in repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens. The C-terminal region of ATM shares homology with catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases).

Upon exposure to ionizing radiation, ATM becomes autophosphorylated and activates downstream signaling pathways regulating cell cycle, apoptosis, and DNA damage repair.
ATM, along with Nbs1 and Chk2, is involved in the E2F1-mediated apoptosis pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.