Thermo Fisher Scientific GCLC Monoclonal Antibody (OTI1A3)
GCLC 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1A3). Western blot, IHC, ICC에 사용 가능. Human, Mouse, Rat 등 다양한 종에 반응. 액상형, 1 mg/mL 농도, PBS/BSA/Glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A3 |
| Immunogen | Full length human recombinant protein of GCLC produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723211 |
Target Information
Glutamate-cysteine ligase (GCLC), also known as gamma-glutamylcysteine synthetase, is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits: a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21.
Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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