Thermo Fisher Scientific NFIX Polyclonal Antibody
NFIX 단백질 검출용 Thermo Fisher Scientific 폴리클로날 항체. Western blot, IHC, ICC 등 다양한 응용 가능. 인간 NFIX 아미노산 116–361 영역 기반 면역원 사용. 비결합형 액상 형태로 4°C 또는 -20°C 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 116–361 of Human NFIX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.76 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2548708 |
Product Specific Information
- Recommended positive controls: 293T
- Predicted reactivity: Mouse (99%), Xenopus laevis (85%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription.
Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene.
NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich, carboxy terminal transactivation domain.
The NF-1 protein recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes.
The NF-1 consensus element has been found in the upstream promoter region of numerous eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and the Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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