Thermo Fisher Scientific H3R8me2a Polyclonal Antibody
H3R8me2a 비대칭 이메틸화 히스톤 H3 R8 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, ELISA, ChIP 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 액상 형태, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic asymmetric dimethylated peptide around R8 of human histone H3 (NP_0035201) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.37 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901615 |
Product Specific Information
- Positive Samples: HeLa, NIH/3T3
- Immunogen sequence: TARKS
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Each nucleosome consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of linker histone H1 with DNA between nucleosomes to form higher-order chromatin structures.
The histone H3 gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails and contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1 and is one of four duplicated histone genes in the cluster, representing the telomeric copy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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