Thermo Fisher Scientific N-cadherin Polyclonal Antibody
Thermo Fisher Scientific의 N-cadherin Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 rabbit IgG 항체입니다. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 액상 형태로 제공됩니다. 세포 간 칼슘 의존성 접착 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 488–587 of human N-Cadherin (NP_0017832) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.16 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901611 |
Product Specific Information
- Positive Samples: HeLa, A-549, 293T, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat heart
- Immunogen Sequence:
VIDVNENPYF APNPKIIRQE EGLHAGTMLT TFTAQDPDRY MQQNIRYTKL SDPANWLKID PVNGQITTIA VLDRESPNVK NNIYNATFLA SDNGIPPMSG
Target Information
N-cadherin is a 140 kDa transmembrane protein belonging to the cadherin family, mediating calcium-dependent intercellular adhesion. Cadherins regulate morphogenetic movements during development and cell surface adhesion through homotypic interactions. N-cadherin’s function depends on its association with the actin cytoskeleton via catenin proteins, regulated by phosphorylation of beta-catenin. It consists of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail, functioning during gastrulation and establishing left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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