Thermo Fisher Scientific Human NEK2, His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

Full length

Tag

His-tag

Molecular weight

54.5 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris, pH 7.5, with 0.05% Triton X-100, 50% glycerol, 2mM DTT, 500mM NaCl, 0.5mM EDTA

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Protein Form: Full Length

Target Information

NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.