Thermo Fisher Scientific H3K9me2 Recombinant Rabbit Monoclonal Antibody (SN07-30)
H3K9me2 표지를 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF에 적합. 인간, 생쥐, 랫트에 반응하며 핵 및 염색체 위치 검출에 활용. 고순도 Protein A 정제 및 안정적 액상 형태 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN07-30 |
| Immunogen | Synthetic peptide within human histone H3 amino acids 1–49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911585 |
Product Specific Information
- Positive Control: HepG2, HeLa, L6, NIH/3T3, human thyroid tissue, human small intestine tissue
- Subcellular Location: Nucleus, Chromosome
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can directly alter chromatin structure or enable binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes multiple modifications, including methylation, acetylation, and phosphorylation, which influence various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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