Thermo Fisher Scientific TLR4 Polyclonal Antibody
Rabbit polyclonal antibody targeting human TLR4 (CD284). Validated for WB, IHC, and ICC/IF applications. Supplied as a liquid, unconjugated form at 1 mg/mL concentration. Recommended storage at 2–8°C short term or -20°C long term. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 15 amino acids near the amino-terminus of human TLR4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151989 |
Product Specific Information
- Suggested positive control: PC-3 cell lysate
- PA5-20021 can be used with blocking peptide PEP-0140
Target Information
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor family, playing a key role in pathogen recognition and activation of innate immunity. These receptors are highly conserved across species and are structurally and functionally similar. TLR4 is expressed abundantly in the placenta and in the myelomonocytic subpopulation of leukocytes. It mediates cellular responses to lipopolysaccharide (LPS) by forming a receptor complex with CD14 and LY96, initiating signaling pathways that lead to cytokine production and immune activation. Mutations in TLR4 can alter LPS responsiveness, and several transcript variants exist, though not all have confirmed protein-coding potential. TLR4-deficient mice demonstrate increased susceptibility to Gram-negative bacterial sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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