Thermo Fisher Scientific Phospho-RPA32 (Ser4, Ser8) Polyclonal Antibody
Phospho-RPA32 (Ser4, Ser8) 폴리클로날 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. DNA 복구 및 복제 관련 단백질 연구용으로 사용됩니다.
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Thermo Fisher Scientific Phospho-RPA32 (Ser4, Ser8) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Dually phosphorylated synthetic peptide representing a portion of Human replication protein A2 (32 kDa) surrounding phosphorylated serines at positions 4 and 8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan.
Target Information
RPA32 is required for DNA recombination, repair, and replication. The activity of replication protein A (RP-A) is mediated by single-stranded DNA binding and protein interactions. It is essential for efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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