Thermo Fisher Scientific Phospho-LCK (Tyr394) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Lck only when phosphorylated at Tyr394, which site historically referenced as Tyr393.

Target Information

LCK is a member of the Src-family tyrosine kinase, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR activation, LCK phosphorylates ITAM motifs on zeta subunits, creating binding sites for ZAP70, which is subsequently phosphorylated and activated. This triggers downstream signaling via adaptor LAT.
LCK is primarily localized to the plasma membrane but also associates with the Golgi apparatus, potentially contributing to Raf activation under weak stimulation. It contains N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains for protein-protein interactions.
LCK plays roles in apoptosis regulation and is associated with diseases such as Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.