Thermo Fisher Scientific p35 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human p35. Recombinant protein control fragment (Product #RP-105673). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p35,uniProtId:Q15078-1,ncbiNodeId:9606,antigenRange:195-298,antigenLength:307,antigenImageFileName:PA5-64865_p35_Q15078-1_Rabbit.svg,antigenImageFileNamePDP:PA5-64865_p35_Q15078-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2663329

Product Specific Information

Immunogen sequence: FITPANVVFLY MLCRDVISSE VGSDHELQAV LLTCLYLSYS YMGNEISYPL KPFLVESCKE AFWDRCLSVI NLMSSKMLQI NADPHYFTQV FSDLKNESGQ EDK

Highest antigen sequence identity to the following orthologs - mouse 100%, rat 100%.

Target Information

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimers disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimers disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.