Thermo Fisher Scientific PYCARD Polyclonal Antibody

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 10 µg/mL

Product Specifications

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.

Target Information

Functions as a key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response, acts as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing pattern recognition receptors such as NLRP2, NLRP3, AIM2, and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not required but facilitates processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may regulate inflammasome adapter function; isoform 3 may inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA, may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity, may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake. May regulate post-transcriptional function of DOCK2 via nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome, involving AP-1, NF-kappa-B, MAPK, and caspase-8 signaling pathways. Modulates NF-kappa-B induction at the IKK complex level by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1, thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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