Invitrogen™

Click-IT™ GlcNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-Azidoacetylglucosamine)

The Click-iT™ GlcNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify자세히 알아보기

The Click-iT™ GlcNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize intracellular O-GlcNAc glycoproteins. In step one, cultured cells are incubated with the azide-modified glucosamine (GlcNAc). The azido-sugar is incorporated into intracellular O-GlcNAc-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then detected with a Click-iT™ Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). The Click-iT™ glycoprotein products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.

사양

그린 기능Less hazardous

제품 유형Labeling Reagent

수량5.2 mg

검출 방법Biotin-based, Fluorescence

배송 조건Room Temperature

제품라인Click-iT™

라벨 또는 염료Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)

Labeling TargetProteins

Unit SizeEach