Thermo Fisher Scientific Click-IT GlcNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-Azidoacetylglucosamine)

Invitrogen™ Click-IT™ GlcNAz Metabolic Glycoprotein Labeling Reagent

(tetraacetylated N-Azidoacetylglucosamine)

The Click-iT™ GlcNAz Metabolic Glycoprotein Labeling Reagent provides the first part of a simple and robust two-step technique to identify and characterize intracellular O-GlcNAc glycoproteins.

1. Step 1: Cultured cells are incubated with the azide-modified glucosamine (GlcNAc). The azido-sugar is incorporated into intracellular O-GlcNAc-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway.
2. Step 2: Through the chemoselective ligation (click reaction) between an azide and an alkyne, the azido-labeled glycoproteins can be detected using a Click-iT™ Glycoprotein Detection Kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne).

The Click-iT™ glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.

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