Thermo Fisher Scientific Click-IT Biotin Protein Analysis Detection Kit

Invitrogen™ Click-IT™ Biotin Protein Analysis Detection Kit

The Click-iT™ Biotin Glycoprotein Detection Kit provides the second part of a simple and robust technique to identify and characterize glycoproteins by Western blot.
In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT™ metabolic labeling reagent or the Click-iT™ Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne.

With this technique, detection sensitivity is in the low femtomole range, and biotin-labeled samples can be detected before or after probing the Western blot with a primary antibody.

주요 특징

• Western blot 기반 glycoprotein 검출 및 특성화
• Azide-alkyne click 반응을 통한 고감도 검출
• Biotin 라벨링으로 높은 신호 감도 제공
• 안전한 저위험성 시약 구성

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