Thermo Fisher Scientific c-Mpl Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Positive control: A549 cell lysate, Raji cell lysate, THP-1 cell lysate, SH-SY5Y, human placenta tissue
• Predicted band size: 71 kDa
• Subcellular Location: Cell membrane, Golgi apparatus, Cell surface

Target Information

In 1990, the oncogene v-mpl was identified from the murine myeloproliferative leukemia virus, capable of immortalizing bone marrow hematopoietic cells from various lineages. In 1992, the human homologue c-mpl was cloned, encoding a protein homologous to the hematopoietic receptor superfamily. Anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation.

The ligand for c-mpl, thrombopoietin, cloned in 1994, was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The c-mpl gene product CD110 is a 635 amino acid transmembrane protein with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs.

TPO-R deficient mice exhibit severe thrombocytopenia, emphasizing the key role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon thrombopoietin binding, CD110 dimerizes, leading to phosphorylation of JAK, STAT, MAPK family kinases, adaptor protein Shc, and the receptor itself.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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