Thermo Fisher Scientific AF4 Polyclonal Antibody
Rabbit polyclonal antibody targeting human proto-oncogene AF4 (AFF1). Suitable for Western blot and IHC applications. Supplied unconjugated in liquid form at 1 mg/mL concentration. Recommended storage at 4°C and use within 1 year.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000–1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human proto-oncogene AF4 (AFF1, UniProt ID: P51825-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, associated with acute leukemias.
Fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor, where AF-4 contributes transcriptional effector properties requiring cell-specific accessory factors.
MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia.
The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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