Thermo Fisher Scientific Krt222 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Krt222. Recombinant protein control fragment (Product #RP-104496). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Krt222,uniProtId:Q8N1A0-1,ncbiNodeId:9606,antigenRange:150-220,antigenLength:295,antigenImageFileName:PA5-62986_Krt222_Q8N1A0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-62986_Krt222_Q8N1A0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2643211

Product Specific Information

Immunogen sequence: YYGCIQGGKK DKKPTTSRVG FVLPSAIINE ISFTTKVPQK YENENVETVT KQAILNGSIV KESTEAHGTI Q

Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 75%.

Target Information

KRT222, also known as KRT222P. KRT222 is a 295 amino acid protein belonging to the intermediate filament family. The gene encoding KRT222P has been listed as a pseudogene, however it has not been established that the protein is not translated, and is therefore treated as a protein coding gene. Existing as two alternatively spliced isoforms, the gene encoding KRT222P maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.