Thermo Fisher Scientific Fascin Monoclonal Antibody (ZM192)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This product is diluted and provided in a ready-to-use formulation.
A recommended positive control tissue is Hodgkin lymphoma, though other positive controls may be used.

The antibody is intended for professional laboratory use in detecting Fascin protein in formalin-fixed, paraffin-embedded tissues using manual qualitative immunohistochemistry (IHC). It should be applied after tumor diagnosis by conventional histopathology.

Fascin Function:
Human Fascin is an actin-bundling protein localized to microspikes and stress fibers, involved in microfilament bundle formation. It is primarily expressed in dendritic cells, while lymphoid, myeloid, and plasma cells are negative. Reed-Sternberg cells in Hodgkin’s lymphoma are positive for Fascin staining. Epstein-Barr virus may induce Fascin expression in B cells. Clone 55K-2 serves as an effective marker for Reed-Sternberg cells.

Usage Recommendations:

• Use with formalin-fixed, paraffin-embedded sections.
• Pretreat deparaffinized tissue with heat-induced or enzymatic epitope retrieval.
• Follow standard IHC staining procedures: primary antibody → link antibody → enzyme complex → chromogenic substrate.
• Interpret results using a light microscope.

Control Tissues:

• Run a positive tissue control with every staining procedure.
• Positive control tissue should include both positive and negative staining cells.
• Use fresh, well-characterized specimens for external controls.
• Weak positive tissues are recommended to detect subtle changes in antibody sensitivity.
• Negative controls (internal or external) should confirm absence of nonspecific staining.

Target Information

Fascin organizes filamentous actin into bundles with a 1:1 actin/fascin ratio and is involved in the assembly of actin filament bundles in microspikes, membrane ruffles, and stress fibers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.