Thermo Fisher Scientific Heat Shock 27kDa Protein 1 Recombinant Rabbit Monoclonal Antibody (HSPB1, 6490R)
Heat Shock 27kDa Protein 1 (HSPB1, HSP27)에 특이적인 Recombinant Rabbit Monoclonal Antibody. Western blot 및 IHC(P) 검증 완료. 인간 반응성, 고순도 Protein A/G 정제, 보존제 없음. 연구용으로 세포 스트레스 및 단백질 폴딩 연구에 적합.
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Thermo Fisher Scientific Heat Shock 27kDa Protein 1 Recombinant Rabbit Monoclonal Antibody (HSPB1, 6490R)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HSPB1, 6490R |
| Immunogen | Recombinant human full-length HSP27 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Heat shock proteins (HSPs) are expressed in response to environmental stress.
HSPB1 (Heat Shock Protein Beta-1, also known as HSP27) acts as a molecular chaperone maintaining denatured proteins in a folding-competent state. It contributes to stress resistance and actin organization.
Through its chaperone activity, HSP27 regulates biological processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in HSPB1 can cause Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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