Thermo Fisher Scientific Heat Shock 27kDa Protein 1 Recombinant Rabbit Monoclonal Antibody (HSPB1, 6490R)
Heat shock 단백질 HSPB1(HSP27)을 인식하는 Rabbit Recombinant Monoclonal 항체. Western blot 및 IHC(P)에서 1-2 µg/mL 농도로 검증됨. HEK293 발현 시스템 기반, Protein A/G 정제, PBS buffer 보관. 세포 스트레스 반응 및 단백질 폴딩 연구에 적합.
- 카탈로그번호
- 3315-RBM6-P1
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HSPB1, 6490R |
| Immunogen | Recombinant human full-length HSP27 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Cells exposed to environmental stress increase expression of heat shock proteins.
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein functioning as a molecular chaperone, maintaining denatured proteins in a folding-competent state.
It contributes to stress resistance and actin organization, and regulates processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in HSPB1 are associated with Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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