Thermo Fisher Scientific PCNA Monoclonal Antibody (1C11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

1C11

Immunogen

KLH conjugated synthetic peptide derived from human PCNA, amino acids 200-250. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PCNA,uniProtId:P12004-1,ncbiNodeId:9606,antigenRange:151-261,antigenLength:261,antigenImageFileName:BSM-2006M_PCNA_P12004-1_House_mouse.svg,antigenImageFileNamePDP:BSM-2006M_PCNA_P12004-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.