Thermo Fisher Scientific His tag Monoclonal Antibody (2D5)
His-tag 단백질 검출용 Thermo Fisher monoclonal antibody (Clone 2D5). Western blot, IHC, ICC/IF 등 다양한 실험에 적용 가능. 고순도 Protein G 정제 및 고안정성 저장 버퍼 구성. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D5 |
| Immunogen | KLH conjugated HHHHHH peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either N- or C-terminal regions of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins for which no specific antibody is available. His-tagged proteins can be purified using Ni²⁺-NTA adsorbent due to strong and selective chelation of histidine clusters to nickel ions.
His tag-specific antibodies, such as clone 2D5, are used to facilitate detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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